P-glycoprotein-mediated herb-drug interaction evaluation between Tenacissoside G...

연구 요약

P-glycoprotein-mediated herb-drug interaction evaluation between Tenacissoside G and paclitaxel.

Biomedical chromatography : BMC 학술지에 발표된 이 연구는 Hu J, Hu Y, Xu L 외 연구팀이 수행하였습니다.

이 연구는 'P-glycoprotein-mediated herb-drug interaction evaluation between Tenacissoside G and paclitaxel.'에 대한 과학적 분석을 제공합니다.

핵심 내용

P-glycoprotein (P-gp)-mediated herb-drug interactions (HDIs) may impact drug efficacy and safety. Tenacissoside G (Tsd-G), a major active component of Marsdenia tenacissima, exhibits anticancer activity. To analyze the effect of Tsd-G on the pharmacokinetics of paclitaxel (PTX), researchers selected 30 Sprague-Dawley (SD) rats, randomized into a solvent control group, a verapamil positive control group, and 20, 40, and 60 mg/kg Tsd-G groups. After seven consecutive days of intraperitoneal injection of verapamil or Tsd-G, a single dose of 6 mg/kg PTX was injected intravenously. Plasma samples were collected at different time points, and proteins were precipitated using a methanol-acetonitrile solution. An ultrahigh-performance liquid chromatography-tandem mass spectrometry method was developed, with docetaxel as an internal standard, and quantified using positive ion multiple reaction monitoring (MRM) mode. This analytical method's specificity, accuracy, precision, recovery, matrix effect, and sample stability meet the requirements for biological sample determination. After Tsd-G administration in rats, the mean residence time of PTX was significantly prolonged. And Tsd-G can stably bind to P-gp by forming hydrogen bonds and inhibiting the expression of P-gp in rat liver. Although the metabolites of PTX were not detected in this study, the above results still indicate the existence of HDIs between Tsd-G and PTX, and P-gp may be the main target to mediate HDIs.

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의사/약사의 전문적 판단을 대체하지 않습니다 (PMID: 39152775)