Cumambrin A from Chrysanthemum boreale attenuates inflammation and oxidative str...

연구 요약

Cumambrin A from Chrysanthemum boreale attenuates inflammation and oxidative stress through TRIF-dependent signaling inhibition and Nrf2/HO-1 pathway activation.

Journal of ethnopharmacology 학술지에 발표된 이 연구는 Moon DH, Son SR, Jang SC 외 연구팀이 수행하였습니다.

이 연구는 'Cumambrin A from Chrysanthemum boreale attenuates inflammation and oxidative stress through TRIF-dependent signaling inhibition and Nrf2/HO-1 pathway activation.'에 대한 과학적 분석을 제공합니다.

핵심 내용

ETHNOPHARMACOLOGICAL RELEVANCE: Chrysanthemum boreale (C. boreale) is applied in traditional Korean and Chinese medicine to treat fever, inflammation, and infectious diseases. Its dried flowers and aerial parts are often prepared as herbal teas or decoctions to reduce fever and inflammation. Phytochemical analysis attributes its medicinal properties with the presence of sesquiterpene lactones, flavonoids, and essential oils. Cumambrin A is one of the sesquiterpene lactones of C. boreale flowers. Despite numerous biological activities of cumambrin A reported, its effects on inflammation in lipopolysaccharide (LPS)-induced model remains to be determined. AIM OF THE STUDY: The anti-inflammatory and antioxidant activities of cumambrin A were investigated along with elucidation of its molecular mechanisms in LPS-induced macrophage model. MATERIALS AND METHODS: Cumambrin A was extracted and isolated from flowers of C. boreale. RAW264.7 macrophages stimulated with LPS were used to examine the effects of cumambrin A on inflammatory mediators such as nitric oxide (NO) and cytokines. Gene and protein expression related to inflammatory signaling were assessed by quantitative real-time PCR and Western blotting. RESULTS: Cumambrin A significantly suppressed NO production (an IC50 value of 10.25 ± 3.68 μM), reactive oxygen species levels, and proinflammatory mediator expressions. Cumambrin A also effectively downregulated the expressions of signal transducer and activator of transcription (STAT)1, STAT3, and interferon regulatory factor 3 (IRF3) phosphorylation, consistent with inhibition of TIR-domain-containing adaptor-inducing interferon-β (TRIF)-dependent signaling. Moreover, cumambrin A upregulated nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) expression, indicating its antioxidant potential. CONCLUSION: Our findings demonstrate that cumambrin A attenuates inflammatory activity and reduces oxidative stress via TRIF-dependent pathway modulation and Nrf2/HO-1 activation, respectively. This study provides the mechanistic basis of cumambrin A's bioactivity, supporting its potential as a promising lead compound derived from C. boreale.

일반인을 위한 해석

구체적인 실천 사항은 담당 의사 또는 약사와 상담하시기 바랍니다.

실천 사항

• 현재 복용 중인 약물이나 영양제에 대해 궁금한 점이 있다면 담당 의사 또는 약사와 상담하시기 바랍니다
• 약물이나 영양제의 용법·용량을 임의로 변경하지 마세요
• 이상 반응이 나타나면 즉시 전문가에게 문의하세요

의사/약사의 전문적 판단을 대체하지 않습니다 (PMID: 41423161)