A Label-Free Nanopore Biosensor for Rapid and Highly Sensitive Detection of Acti...

연구 요약

A Label-Free Nanopore Biosensor for Rapid and Highly Sensitive Detection of Actinomycin in Human Serum.

ACS measurement science au 학술지에 발표된 이 연구는 Zhao Y, Zheng H, Munusamy S 외 연구팀이 수행하였습니다.

이 연구는 'A Label-Free Nanopore Biosensor for Rapid and Highly Sensitive Detection of Actinomycin in Human Serum.'에 대한 과학적 분석을 제공합니다.

핵심 내용

Actinomycin D (ActD), a potent anticancer drug, binds specifically to DNA at GpC-rich sites through intercalation and minor groove interactions. Detecting trace levels of ActD in complex biological fluids, such as human serum, is crucial for clinical, environmental, and pharmaceutical applications. Herein, we report a label-free detection method using an engineered α-hemolysin (α-HL) nanopore. In this assay, ActD binds to a ssDNA probe (GC2), enriched with GpC sites, forming GC2-ActD complexes that generate current modulations with distinct signatures from those of GC2 alone. This sensor achieves high sensitivity, with a detection limit (LOD) of 1.20 nM, and a broad linear range of 2.5-250 nM. The sensor selectivity and the effects of metal ions and salt concentration on its performance were further evaluated. Moreover, this sensor enabled ActD detection in complex human serum samples with high selectivity. Overall, this DNA-based nanopore platform offers a powerful, label-free tool for drug screening and discovery.

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의사/약사의 전문적 판단을 대체하지 않습니다 (PMID: 41727368)

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