Anthocyanin-rich polyphenols from Hibiscus syriacus activate autophagy to revers...

연구 요약

Anthocyanin-rich polyphenols from Hibiscus syriacus activate autophagy to reverse polystyrene microplastic-induced osteogenic dysfunction.

Phytomedicine : international journal of phytotherapy and phytopharmacology 학술지에 발표된 이 연구는 Kavinda MHD, Karunarathne WAHM, Lee KT 외 연구팀이 수행하였습니다.

이 연구는 'Anthocyanin-rich polyphenols from Hibiscus syriacus activate autophagy to reverse polystyrene microplastic-induced osteogenic dysfunction.'에 대한 과학적 분석을 제공합니다.

핵심 내용

BACKGROUND: The environmental accumulation of microplastics (MPs), defined as plastic particles ≤5 mm, has emerged as a critical global health concern, particularly owing to their potential to impair skeletal development and bone homeostasis. PURPOSE: This study aimed to determine whether anthocyanin-rich polyphenols extracted from Hibiscus syriacus L. (AH) could restore osteogenesis impaired by polystyrene MPs (PS-MPs) by targeting osteoblast senescence and autophagy regulation. METHODS: MC3T3-E1 preosteoblasts were exposed to PS-MPs followed by treatment with AH, and osteogenic recovery was evaluated via alkaline phosphatase (ALP) activity, calcium mineralization, and expression of osteogenic markers (RUNX2, SP7, and ALP) over 14 days. Senescence-associated secretory phenotype (SASP) markers (Tnf, Mmp13, Il6, and Il1b), key senescence regulators (p21, p27, MMP13, and Lamin B1), and autophagy markers (p62 and LC3B) were assessed using RT-qPCR, western blotting, and immunofluorescence. Zebrafish larvae were employed as an in vivo model to evaluate vertebral mineralization using calcein staining, as well as the expression of SASP osteogenic genes. RESULTS: PS-MP exposure markedly reduced ALP activity, mineral deposition, and osteogenic gene/protein expression, while elevating senescence markers, indicative of severe osteoblast dysfunction. AH treatment significantly reversed these effects, effectively restoring ALP activity and mineralization. Furthermore, AH activated autophagy, as evidenced by increased LC3B and reduced p62 expression. In zebrafish, AH ameliorated PS-MP-induced defects in vertebral mineralization, reduced skeletal malformations, and upregulated osteogenic genes. CONCLUSION: AH mitigates PS-MP-induced osteogenic dysfunction through the dual modulation of osteoblast senescence and autophagy activation, supporting its potential as a natural therapeutic strategy against MP-related skeletal disorders.

일반인을 위한 해석

구체적인 실천 사항은 담당 의사 또는 약사와 상담하시기 바랍니다.

실천 사항

• 현재 복용 중인 약물이나 영양제에 대해 궁금한 점이 있다면 담당 의사 또는 약사와 상담하시기 바랍니다
• 약물이나 영양제의 용법·용량을 임의로 변경하지 마세요
• 이상 반응이 나타나면 즉시 전문가에게 문의하세요

의사/약사의 전문적 판단을 대체하지 않습니다 (PMID: 41421288)